DNA polymorphisms at α-Gpdh locus of Drosophila melanogaster in Korean population

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Restriction site variation in 18-kb region including the <i>Gpdh</i> (<i>sn</i>-glycerol-3-phosphate dehydrogenase) locus has been assessed in 27 isofemale lines, 12 lines from the Chounan population and 15 lines from the Pusan population in Korea. Of the 25 restriction sites that were scored, 5 sites were polymorphic (20.0%): <i>Pst</i>I (-2.35), <i>Xba</i>I (-1.7), <i>Hin</i>dIII (0.0), <i>Hin</i>dIII (+0.25), and <i>Pst</i>I (+5.7). Two insertions (I) and three deletions (D) were detected. All the restriction sites and length polymorphisms were located in introns and outside regions of the <i>Gpdh</i> structure gene, the area from -7.0 site to -5.5 site. The proportion of segregating nucleotides, <i>p</i>, for all 27 lines was 0.019 and the estimated heterozygosity per nucleotide pair, was 0.013. Sixteen kinds of restriction map haplotypes of all lines analyzed were detected. Few lines were identical at the restriction map level. The estimated haplotype diversity, <i>h</i>, was 0.968. The haplotype diversity (0.997) of <i>Gpdh</i><sup>FF</sup> bearing chromosomes was slightly higher than that (0.982) of <i>Gpdh</i><sup>SS</sup> chromosomes. Analysis for a linkage disequilibria between the polymorphism also showed that <i>Hin</i>dIII (+0.25) vs. I (a) and <i>Pst</i>I (-2.35) vs. D (a) have relatively high level of the linkage disequilibria. There were no significant relationship between restriction site polymorphism and GPDH activity in 27 second-chromosome lines; however, there was an apparent difference in the effect of length variation on GPDH activity. The activity of deletion-bearing lines was lower than that of deletion-free lines, while there was no effect of insertion on activity. Duplication of <i>Gpdh</i> was observed in about one-third of the 27 isofemale lines. The duplication-bearing lines in both populations had higher levels of GPDH activity and GPDH CRM than duplication-free lines. In a further investigation of the duplication structure, the duplication of Korean populations was shown to have a similar structure to other populations investigated (Symonds and Gibson, 1992; Takano et al., 1989).<br>

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  • Genes & genetic systems

    Genes & genetic systems 73(4), 227-235, 1998-08

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003762833
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4598857
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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