Genetic mapping of RAPD markers linked to the densonucleosis refractoriness gene, nsd-1, in the silkworm, Bombyx mori

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著者

    • SHIMADA Toru
    • Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • OHBAYASHI
    • Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
    • OSHIKI Toshikazu
    • Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology

抄録

In the silkworm, <i>Bombyx mori</i>, nonsusceptibility to <i>B. mori</i> densonucleosis virus type-1 (BmDNV-1) is controlled by a recessive gene, <i>nsd-1</i> (nonsusceptibility to DNV-1), located on the twenty-first chromosome. We investigated genetic linkage between five random amplified polymorphic DNA (RAPD) markers and the +<sup>nsd-1</sup> gene. Initially, we constructed the CSD-1 strain (<i>nsd-1</i>/+) which is congenic to strain C137 (<i>nsd-1</i>/ <i>nsd-1</i>) for the twenty-first chromosome, starting with a female of C137 and a male of strain J137 (+<sup>nsd-1</sup>/+<sup>nsd-1</sup>). For the crossing over experiment, a female of C137 was crossed with a male (<i>nsd-1</i>/+) of CSD-1. Segregation analysis showed that the most closely linked RAPD marker mapped 3.0 cM distant from +<sup>nsd-1</sup>. A more specific marker for +<sup>nsd-1</sup> was made by converting this RAPD band into a sequence characterized amplified region (SCAR) using a series of newly designed primer pairs based on its DNA sequence.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(4), p.237-242, 1998-08

    The Genetics Society of Japan

参考文献:  37件中 1-37件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10003762857
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4598858
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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