Identification of novel random amplified polymorphic DNAs (RAPDs) on the W chromosome of the domesticated silkworm, Bombyx mori, and the wild silkworm, B. mandarina, and their retrotransposable elementrelated nucleotide sequences.

DOI Web Site Web Site PubMed 9 Citations 60 References
  • Abe Hiroaki
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Kanehara Mariko
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Terada Tomoko
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Ohbayashi Fumi
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Shimada Toru
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Kawai Shinya
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Suzuki Masataka
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Sugasaki Toshiyuki
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • Oshiki Toshikazu
    Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology

Bibliographic Information

Other Title
  • Identification of novel random amplifie

Search this article

Abstract

Genomic DNAs were compared between males and females of the domesticated silkworm, Bombyx mori, strains C108, C137, J137, p50, and WILD-W (constructed by crossing a wild silkworm, B. mandarina, female with a male of strain C108) by polymerase chain reaction (PCR) with 700 arbitrary 10-mer primers. Four female-specific RAPDs (W-Kabuki, W-Samurai, W-Kamikaze, and W-Yamato) were found. The sex chromosome formulas of B. mori and B. mandarina are ZW (XY) for the female and ZZ (XX) for the male. The four female-specific RAPDs are assumed to be derived from the W chromosome because the other chromosomes are shared by both sexes. A computer search for deduced amino acid sequences of these four RAPDs revealed that all of them showed homology to previously reported amino acid sequences encoded in known retrotransposable elements from various organisms.<br>

Journal

Citations (9)*help

See more

References(60)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top