The molecular basis of the instability of a crp^- mutation in Escherichia coli

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We have described a rapid spontaneous conversion in the stationary phase of <i>Escherichia coli</i> strain DOO (<i>crp</i> <sup>-</sup>) cells as a whole population to <i>crp</i><sup>+</sup> state (Sugino and Morita, 1994). In this paper we have tried to elucidate the molecular basis of this unidirectional conversion by cloning and sequencing of the <i>crp</i> gene in their <i>crp</i><sup>+ </sup>and <i>crp</i><sup>-</sup> states. We have found that in the original <i>crp</i><sup>-</sup> strain, an IS<i>2</i> element has been inserted between its original promoter and the coding region of the <i>crp</i> gene in the so-called orientation II (Ahmed et al., 1981), accompanied by an 11 bp deletion. Unexpectedly, the <i>crp</i> <sup>+</sup> "revertants" derived from the <i>crp</i><sup>-</sup> mutant had no difference in sequence from the <i>crp</i><sup>-</sup>, either in the coding or the regulatory region. This suggests that a change at another locus, such that this change somehow activates the expression of the <i>crp</i> gene to the level of a normal <i>crp</i> <sup>+</sup>, is responsible for the apparent reversion from <i>crp</i> <sup>-</sup> to <i>crp</i><sup>+</sup>.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 73(6), 407-413, 1998-12-01

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10003763512
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    4682013
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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