CATHODIC ELECTROCHEMILUMINESCENCE OF LUMINOL ENHANCED BY ANTIBODY-ANTIGEN REACTION

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The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemilumineseent intensity of luminol resulted from antigen-antibody reactions. The cathodic clectrochemiluminescences of lurninol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic clectruchemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous inummoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presenee of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while lummol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.

収録刊行物

  • Journal of chemical engineering of Japan

    Journal of chemical engineering of Japan 29(5), 851-857, 1996-10-20

    The Society of Chemical Engineers, Japan

参考文献:  18件中 1-18件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10003815494
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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