Cathodic Electrochemiluminescence of Luminol Enhanced by Antibody-Antigen Reaction.
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- Yoshimi Yasuo
- Department of Chemical Engineering, Waseda University
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- Haramoto Hirotaka
- Department of Chemical Engineering, Waseda University
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- Miyasaka Takehiro
- Department of Chemical Engineering, Waseda University
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- Sakai Kiyotaka
- Department of Chemical Engineering, Waseda University
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The purpose of the present work is to characterize the cathodic electrochemiluminescence of luminol with a view to its application in continuous immunoassay. The authors designed a method for continuous antibody determination from the change in electrochemilumineseent intensity of luminol resulted from antigen-antibody reactions. The cathodic clectrochemiluminescences of lurninol-labeled antigens in hydrogen peroxide solution were enhanced by the presence of the respective antibodies. Cathodic clectruchemiluminescence of a luminol-labeled antibody was also enhanced by the presence of antigen. The enhancement of electrochemiluminescence may be useful in homogeneous inummoassay of antibodies or antigens. Analysis of a batchwise reaction suggested that the presenee of antibody increases the quantum yield of electrochemiluminescence of luminol-labeled antigen. And analysis of the electrolytic current of luminol and hydrogen peroxide at indium-tin oxide (ITO) cathode revealed that hydrogen peroxide was electrochemically active at the cathode, while lummol was inert. These results suggest that cathodic electrochemiluminescence of luminol or luminol-labeled proteins is triggered by hydroxide radical produced from hydrogen peroxide at the ITO cathode.
収録刊行物
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- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
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JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 29 (5), 851-857, 1996
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204566018816
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- NII論文ID
- 10003815494
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- NII書誌ID
- AA00709658
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- COI
- 1:CAS:528:DyaK28XmsVSmsL4%3D
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- ISSN
- 18811299
- 00219592
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可