BIOAFFINITY SEPARATION OF CONCANAVALIN A IN REVERSE MICELLAR SYSTEM COMPOSED OF AOT/BUTANOL OR NON-IONIC SURFACTANT

この論文にアクセスする

この論文をさがす

著者

抄録

Selective separation of concanavalin A (Con A) using alkylglucoside (AGn) as an affinity ligand was achieved by two different methods in reverse micellar systems. The first method used the sodium bis(2-ethylhexyl)sulfosuccinate (AOT)/butanol/iso-octane system, in which Con A was extracted effectively when AGn was added to the system but was not extracted at all without AGn. Selective extraction was attained in three steps. In the first step, reverse micelles composed of AOT and alcohol without AGn were used. Proteins extractable to these reverse micelles were removed from the aqueous phase. In the second extraction step, Con A was selectively extracted to the reverse micellar phase with AGn. Backward extraction was successfully performed using buffer solutions containing glucose. The problem of this method was the precipitation of Con A at the liquid/liquid interface presumably due to the denaturation of Con A by AOT. The second method used the reverse micellar system composed of tetra-oxyethylenedecylether. This reverse micellar system had no ability to extract water-soluble proteins without an affinity ligand, whereas Con A could be extracted selectively with high overall efficiency when AGn was added to the system. Rapid backward extraction was performed using a buffer solution containing glucose or methyl-<I>α</I>-glucoside by adding ethanol or butanol.

収録刊行物

  • Journal of chemical engineering of Japan

    Journal of chemical engineering of Japan 29(6), 982-989, 1996-12-01

    The Society of Chemical Engineers, Japan

参考文献:  18件中 1-18件 を表示

被引用文献:  2件中 1-2件 を表示

各種コード

  • NII論文ID(NAID)
    10003815744
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00219592
  • NDL 記事登録ID
    4109686
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ