Study on Rapid Analysis for Microcystin.

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  • NISHIMURA Kazuyuki
    Department of Architecture and Civil Engineering, Toyohashi University of Technology
  • KAWAMOTO Hideo
    Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd.
  • ITOH Mitsuaki
    Institute of Environmental Ecology, Shin-Nippon Meteorological & Oceanographical Consultant Co. Ltd.
  • KUNIKANE Shoichi
    National Institute of Public Health

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Other Title
  • ミクロシスチンの簡易測定手法に関する研究
  • ミクロシスチン ノ カンイ ソクテイ シュホウ ニ カンスル ケンキュウ

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Abstract

The fast analysis of microcystin in environmental water was examined by using a protein phosphatase 2A inhibition assay. The heat treatment method was developed for rapid extraction of microcystin from Microcystis cells.<br>Quantitative analysis of microcystin by the protein phosphatase 2A inhibition assay required two hours. The assay was accurate and reproducible. The cultured Microcystis suspension was submitted to the protein phosphatase 2A inhibition assay as well as commercial ELISA assay kit with similar results. But sensitivity of this assay was not so good as the ELISA assay kit.<br>The microcystins were extracted from Microcystis cells by heat conditions such as temperature of 50-60 C° and heating time of more than 20 minutes. The heat treatment with α-amylase, cellulase and lysozyme was slightly facilitated to extraction of it.<br>Uses of the protein phosphatase 2A inhibition assay with the heat treatment method facilitate investigation of microcystin in natural water.

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