Tissue Based Enzymatic Determination of ATP in Human Erythrocyte Hemolysate Combined with Spectrophotometric Method

• Makoto Kataoka

  Laboratory of Analytical Chemistry, Department of Chemistry, University of TsukubaTsukuba

• Norio Teshima

  Laboratory of Analytical Chemistry, Department of Chemistry, University of TsukubaTsukuba

• Makoto Kurihara

  Laboratory of Analytical Chemistry, Department of Chemistry, University of TsukubaTsukuba

• Katsuhiko Kuwa

  College of Medical Technology and Nursing, University of TsukubaTsukuba

• Takuji Kawashima

  Laboratory of Analytical Chemistry, Department of Chemistry, University of TsukubaTsukuba

抄録

A potato tissue based enzymatic determination of ATP combined with spectrophotometric detection is proposed. ATP can be hydrolyzed to give AMP via ADP and two moles of orthophosphate by apyrase (EC 3.6.1.5) contained in the potato tissue. The phosphate released is determined spectrophotometrically based on molybdenum blue formation (λmax = 712 nm). A linear calibration graph is obtained over the range 1.3 × 10−6–1.3 × 10−5 mol dm−3 ATP with a detection limit of 1.5 × 10−7 mol dm−3 (S/N = 3). The proposed method was successfully applied to the determination of ATP in human erythrocyte hemolysate.

収録刊行物

• Chemistry Letters

  Chemistry Letters 28 (6), 491-492, 1999-06-01

  Oxford University Press (OUP)

キーワード

• General Chemistry

詳細情報

• CRID: 1360283694086386432
• NII論文ID: 10004486167
• NII書誌ID: AA00603318
• DOI: 10.1246/cl.1999.491
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/28/6/491/56076863/cl.1999.491.pdf
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