Quantitative Chromosome Map of Arabidopsis thaliena L. by Imaging Methods.

DOI Web Site 被引用文献8件 参考文献26件
  • Ito Mikako
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • Ohmido Nobuko
    Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station
  • Akiyama Yukio
    Laboratory of Rice Genetic Engineering, Hokuriku National Agricultural Experiment Station Japan Science and Technology Corporation
  • Fukui Kiichi
    Department of Biotechnology, Graduate School of Engineering, Osaka University

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Arabidopsis thaliana L. has 10 chromosomes that are small and similar in morphology. Molecular cytogenetics and image analyses were applied to characterize the somatic chromosomes. Prometaphase chromosomes were quantitatively analyzed based on their patterns of condensation, because they show a prominent uneven condensation along the chromosomes. The total length of the haploid prometaphase chromosomes is 16.5 μm, which is about 1.8 times the size of the metaphase chromosomes. We also detected the 45S (18S-5.8S-25S) and 5S rDNA loci by fluorescence in situ hybridization (FISH) and determined their locations on the chromosomes by the imaging methods. As a result, a quantitative chromosome map based on the condensation pattern was developed for the first time in Arabidopsis with the locations of rDNAs by using imaging methods. It is now possible to localize genes precisely on this map.

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