Electrochemical Detection of Biotin Using an Interaction between Avidin and Biotin Labeled with Ferrocene at a Perfluorosulfonated Ionomer Modified Electrode.
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- TANAKA Shunitz
- Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University
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- YOSHIDA Kazuyo
- Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University
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- KURAMITZ Hideki
- Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University
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- SUGAWARA Kazuharu
- Kitami Institute of Technology
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- NAKAMURA Hiroshi
- Division of Material Science, Graduate School of Environmental Earth Science, Hokkaido University
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Abstract
The electrochemical behavior of biotin labeled with ferrocene was investigated at a perfluorosulfonated ionomer (Nafion®) film-coated glassy carbon electrode (GCE). The electrode response of the labeled biotin (LB) was improved by about fifty-fold in sensitivity by using a Nafion®-coated GCE, compared with a plain GCE. It was found by a solid-phase avidin-biotin binding assay that the labeling with ferrocene hardly affected the affinity of a biotin moiety in the labeled biotin to avidin. The avidin-biotin interaction was estimated from the change of the electrode response caused by the formation of a complex with avidin and the labeled biotin. Furthermore, the detection of biotin was performed by a competitive reaction between the labeled biotin and unlabeled biotin for the limited binding sites of avidin.
Journal
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- Analytical Sciences
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Analytical Sciences 15 (9), 863-866, 1999
The Japan Society for Analytical Chemistry
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Keywords
Details 詳細情報について
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- CRID
- 1390001204254556032
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- NII Article ID
- 130004440142
- 10004707477
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1MXlvV2qtL0%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4868078
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed