Chemiluminescence Flow Biosensor System for Cholesterol with Immobilized Reagents.

• HUANG Yuming

  Department of Chemistry, Nanjing University

• ZHANG Chao

  Department of Chemistry, Shaanxi Normal University

• ZHANG Zhujun

  Department of Chemistry, Shaanxi Normal University Department of Chemistry, Southwest Normal University

抄録

A chemiluminescence biosensor combined with flow injection analysis has been developed for determining cholesterol. Cholesterol oxidase was immobilized onto amine-modified silica gel via glutaraldehyde activation and packed in a column. The analytical reagents, including luminol and ferricyanide, were electrostatically coimmobilized on an anion-exchange column. Cholesterol was sensed by the CL reaction between hydrogen peroxide released from the enzymatic reaction and luminol and ferricyanide, which were released from a column with immobilized reagents by elution. The calibration graph was linear over the range 5×10^-6 to 1×10^-4 g ml^-1 and the detection limit was 5×10^-6 g ml^-1. The pro-posed method has been successfully applied to the determination of cholesterol in human serum.

収録刊行物

• Analytical Sciences

  Analytical Sciences 15 (9), 867-870, 1999

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390001204257246976
• NII論文ID: 130004440156
• NII論文ID: 10004707502
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.15.867
• COI: 1:CAS:528:DyaK1MXlvV2qtLo%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 4868086
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4868086
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.15.867.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.15.867/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/15/9/15_9_867/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000047469
• 本文言語コード: en
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