Microscopic Image Resolved by Fluorescence Lifetime of a Fluorescent Probe Incorporated in Cultivated Cancer Cells
We measured the fluorescence lifetime by a new method (Itoh et al., 1997a) from cross-correlation signals at an optical point on the fluorescence image of human melanoma cultivated cells stained by the DNA probe acridine orange (AO). As the lifetime values from the phase-delay of the cross-correlation signals were almost the same as the modulation by a framing camera, the method was applicable to taking ultra fast time-resolved fluorescent images. The obtained shorter lifetime (1.1-2.3 nsec)-resolved image of the red fluorescent region was about the same as that from the fluorescence lifetime obtained in 1.98 nsec by the laser flash photolysis method. lt was considered that the shorter lifetime region in the cytoplasm particle was in the cytoplasm that included single-stranded RNA of living melanoma cells.
Bioimages 8(1), 1-3, 2000-03