Imaging of Injection and Separation Processes of DNA on a Microfabricated Capillary Electrophoresis Device

• Ueda Masanori

  Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima Japan Foundation for Aging and Health

• Abe Hirohisa

  Technology Research Laboratory, Shimadzu Corporation

• Kuyama Hiroki

  Technology Research Laboratory, Shimadzu Corporation

• Nakanishi Hiroaki

  Technology Research Laboratory, Shimadzu Corporation

• Arai Akihiro

  HPLC Business Department, Analytical Instruments Division, Shimadzu Corporation

• Baba Yoshinobu

  Department of Medicinal Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokushima CREST, Japan Science and Technology Corporation

Abstract

A laser-induced fluorescence detection system coupled with a highly sensitive silicon intensified target (SIT) video camera has been successfully applied to the imaging of a band for DNA fragment labeled with fluorescence dye and the visualizing of a separation process in the microchannel fabricated on a chip. We demonstrated that the imaging of the sample injection process is a powerful method for optimizing the electric potentials. Moreover, in microfabricated capillary electrophoresis (μ-CE) devices, continuous observation of the sample separation process is available. This direct imaging method is useful for the study of separation dynamics on μ-CE devices.

Journal

• bioimages

  bioimages 7 (4), 157-161, 1999

  Bioimaging Society

Keywords

• μ-TAS
• μ-CE
• Lab-on-Chip
• capillary electrophoresis

Details

• CRID: 1391975276374339328
• NII Article ID: 10004718514
• NII Book ID: AA11084187
• DOI: 10.11169/bioimages.7.157
• ISSN: 09192719
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed