Genetic Variability of DNA Fingerprinting in 5 strains of Red Sea Bream, Pagrus major.

• TAKAGI Motohiro

  高知大学農学部

• MIKITA Kazuhiko

  高知大学農学部

• SEKI Shingo

  高知大学農学部

• TANIGUCHI Nobuhiko

  高知大学農学部

Bibliographic Information

Other Title

• ＤＮＡフィンガープリントによる養殖マダイ５系統の遺伝変異保有量

Abstract

Genetic variability of five strains of red sea bream was investigated by DNA fingerprinting (DNA-FP) using samples of one non-selected strain (S1), 3 strains of growth rate-selected fish (S2 S3, S4), one strain of hybridized and growth-rate selected fish (S5), and wild population. Extracted DNA of these strains were digested by Hinf I restriction enzyme and hybridized with 33.15 and YNZ 22 probes. Band sharing indices (BSI) were calculated from the banding pattern of DNA-FP.<BR>Mean BSI of DNA-FP detected with the 33.15 multilocus probe were relatively high in S2 (0.382) and S3 (0.301), and relatively low in S1 (0.226), S4 (0.166) and S5 (0.206) comparing with the very low value of the wild population (0.120) . There were no differences in BSI values obtained by the two probes.<BR>These results were agreed well with allozyme analysis, and it is concluded that DNA-FP can be used as a marker for monitoring change in genetic variability in artificially propagated fishes for aquaculture.

Journal

• Aquaculture Science

  Aquaculture Science 43 (4), 491-497, 1995

  Japanese Society for Aquaculture Science

Keywords

• マダイ
• 養殖マダイ系統
• DNAフィンガープリント
• 遺伝変異保有量

Details

• CRID: 1390282679694610944
• NII Article ID: 10004769992
• NII Book ID: AN00124667
• DOI: 10.11233/aquaculturesci1953.43.491
• ISSN: 21850194
• ISSN: 03714217
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030570972
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • CiNii Articles
• Abstract License Flag: Disallowed