二次元泳動地図におけるスポット出現時期によるサクラマス (ヤマメ) の胚発生の特徴づけ: 11個のタンパク質スポットのN末端配列の決定

DOI 日本農学文献記事索引 Web Site 参考文献28件
  • 金谷 重彦
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 工藤 喜弘
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 平松 良次
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 斎藤 実
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 清野 明
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 氏家 義史
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 佐藤 典子
    Department of Electrical Information Engineering, Faculty of Engineering, Yamagata University
  • 桂 和彦
    Yamagata Prefectural Inland Water Fisheries Experiment Station Yonezawa

書誌事項

タイトル別名
  • Characterization of Embryogenic Stages Based on Two-Dimensional Electrophoresis for Oncorhynchus masou Yamame: Determination of N-Terminal Sequences of Eleven Spots.
  • Characterization of embryogenic stages

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抄録

To characterize embryogenic stages of Yamame Oncorhynchus masou, we analyzed the total protein constituents in the whole embryo, during the 35-day period from fertilization to hatching on a series of 2-dimensional gels and indexed 706 spots. We determined N-terminal sequences for the 11 spots that were presumably stage-specific and carried out a homology search by using FASTA program for non-redundant database in Genome Net in Japan (version 3.0t76) consisting of 302634 protein sequences. The results showed that the N-terminal sequences of 9 spots (ID=7, 8, 423, 502, 512, 522, 528, 570, 601) contained either very similar or identical to the partial sequences of vitellogenin for Oncorhynchus mykiss. Of the nine, spots 502 and 512 appeared on day 6 after fertilization and continued to be visible until swim-up stage in normal embryos. In case of haploid embryos induced by an ultraviolet system (3600 erg/mm2), the two spots appeared on days 8-10 after fertilization. In unfertilized embryos, the two spots did not appear on the maps. The result suggested that spots 502 and 512 might be useful for determining whether the embryo is developing normally. The N-terminal sequences of spots 58 and 324, which appeared on day 23 after fertilization were very similar to a partial sequence of apolipoprotein (ApoA-I) and identical to a partial sequence for α-tropomyosin, respectively. In a haploid embryo on day 23, spot 324 was detected very faint suggesting its usefulness for determining whether the embryo is developing normally.

収録刊行物

  • 水産増殖

    水産増殖 46 (3), 343-354, 1998

    日本水産増殖学会

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