The trpF Nucleotide Sequence and Its Promoter Analysis in Pseudomonas aeruginosa

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Abstract

The nucleotide sequence of the gene for the phosphoribosyl anthranilate isomerase (PRAI) (<i>trpF</i>) in <i>Pseudomonas aeruginosa</i> was determined. The gene encoded a polypeptide of 211 amino acid residues and its initiation codon was UUG. Northern hybridization analysis showed only one transcript for <i>trpF</i> (about 700 nucleotides). Primer extension analysis revealed three transcription start sites which were at positions 52, 55 and 57 bases upstream of the initiation codon. A highly GC-rich 9-base-pair inverted repeat with 5-base-pair spacing was found upstream of a putative Shine-Dalgarno sequence. This inverted repeat could form a 23-nucleotide stem-loop structure in the transcript. Deletion of the inverted repeat slightly decreased the promoter activity of <i>trpF</i>. The presence of tryptophan had no effect on transcription of <i>trpF</i>.

Journal

  • MICROBIOLOGY and IMMUNOLOGY

    MICROBIOLOGY and IMMUNOLOGY 40(2), 107-114, 1996-02-20

    Center For Academic Publications Japan

References:  34

Cited by:  1

Codes

  • NII Article ID (NAID)
    10004787088
  • NII NACSIS-CAT ID (NCID)
    AA00738350
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03855600
  • Data Source
    CJP  CJPref  J-STAGE 
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