Nucleotide Sequence of the SrRNA Gene and Phylogenetic Analysis of Trichomonas tenax

• FUKURA Katsuhisa

  Department of Oral Microbiology, Showa Unversity School of Dentistry

• YAMAMOTO Ayako

  Department of Oral Microbiology, Showa Unversity School of Dentistry

• HASHIMOTO Tetsuo

  The Institute of Statistical Mathematics

• GOTO Nobuichi

  Department of Oral Microbiology, Showa Unversity School of Dentistry

抄録

The small subunit ribosomal RNA (SrRNA) gene of Trichomonas tenax ATCC30207 was amplified by PCR and the 1.55-kb product was cloned into plasmid vector pUC18. Four clones were isolated and sequenced. The insert DNAs were 1, 552bp long and their G+C contents were 48.1%; three of them had exactly the same DNA sequences and one had only one nucleotide change. A representative SrRNA sequence was analyzed and a phylogenetic tree was estimated by the neighbor joining (NJ) method. Among the protists examined, T. tenax was placed as the closest relative of Tritrichomonas foetus, as expected from the traditional taxonomy. The total homology between the two SrRNA sequences was 89.2%.

収録刊行物

• Microbiology and immunology

  Microbiology and immunology 40 (3), 183-188, 1996-03-20

  財団法人 学会誌刊行センター

キーワード

• Trichomonas tenax
• SrRNA
• Phylogeny

詳細情報

• CRID: 1573668923992498048
• NII論文ID: 10004787360
• NII書誌ID: AA00738350
• ISSN: 03855600
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK28XitFeksr4%3D&md5=f1150469de67831eb6ba721676080be0
• Web Site: https://search.jamas.or.jp/link/ui/1996166836
• 本文言語コード: en
• データソース種別:
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