Cloning and Sequencing of the Central Region of the Flagellin Gene from the Gram-Positive Bacterium Clostridium tyrobutyricum ATCC 25755

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Author(s)

    • Bedouet Laurent BEDOUET Laurent
    • Universite de Bretagne Occidentale, Institut Universitaire de Technologie, Laboratoire Universitaire de Microbiologie Appliquee de Quimper
    • ROBREAU Georges
    • Universite de Bretagne Occidentale, Institut Universitaire de Technologie, Laboratoire Universitaire de Microbiologie Appliquee de Quimper

Abstract

The purpose of this study was to sequence the central part of the coding region of the <i>Clostridium tyrobutyricum</i> flagellin gene to improve the immunoenzymatic counting of cells after milk filtration. The coding region was amplified by PCR, and the amplified products were cloned. A DNA sequence analysis of positive clones gave us 1, 131 nucleotides with a partial calculated flagellin molecular mass of 40, 143 Da. The flagellar filament protein sequence exhibited high levels of homology to sequences of flagellin protein from other bacteria in both N- and C-terminal parts, but little homology in the central domain. A PCR-restriction fragment length polymorphism analysis of amplified <i>C. tyrobutyricum</i> flagellin gene products confirmed the variability of the central domain. The flagellin mRNA was determined to be 1.1kb in size, which suggests a monocistronic mRNA. Furthermore, the deduced protein flagellin contains eleven potential <i>N</i>-glycosylation sites and one sequence rich in serine, which could be modified by <i>O</i>-glycosylation.

Journal

  • MICROBIOLOGY and IMMUNOLOGY

    MICROBIOLOGY and IMMUNOLOGY 43(1), 1-8, 1999-01-20

    Center For Academic Publications Japan

References:  37

Codes

  • NII Article ID (NAID)
    10004787902
  • NII NACSIS-CAT ID (NCID)
    AA00738350
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03855600
  • NDL Article ID
    973193
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z53-M387
  • Data Source
    CJP  NDL  J-STAGE 
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