Repetitive Sequence of Leptospira interrogans Serovar Icterohaemorrhagiae Strain Ictero No.1:A Sensitive Probe for Demonstration of Leptospira interrogans Strains

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Abstract

A 4.8-kilobase (kb) repetitive sequence element generated with <i>Kpn</i>I digestion was cloned from the <i>Leptospira interrogans</i> serovar icterohaemorrhagiae strain Ictero No. 1. The sequence, repeated in tandem, was located on the 280-kb fragment between the <i>Fse</i>I and <i>Asc</i>I sites on the chromosome by hybridization using the 4.8-kb fragment as a probe. We cloned the fragment containing the element for the Ictero No. 1 strain in a lambda EMBL3 bacteriophage DNA, and one out of 5 clones was sequenced. Within the sequenced 9-kb segment that partially repeated, 9 putative open-reading frames and 2 transfer RNA genes, for alanine and isoleucine, were identified. A similarity search for the products deduced from the sequenced data revealed that the repeated sequence includes both beta-oxidation enzymes, acyl-CoA dehydrogenase and enoyl-CoA hydratase, and hydroxythiazole kinase protein homologues. Hybridization experiments against different leptospiral strains using the element as a probe showed a similar sequence in the strains of <i>L. interrogans</i> and <i>L. kirschneri</i>, but not in any strains of <i>L. borgpetersenii, L. weillii, L. meyeri</i> or <i>L. biflexa</i>. Results indicated that the highly repeated element in the Ictero No. 1 strain exists as a well conserved sequence, though at a moderate level of repetition, in certain strains of <i>L. interrogans</i> and <i>L. kirschneri</i>. PCR amplification targeting the repetitive element was successful and indicated that the procedure provides a sensitive and specific probe to detect leptospires.

Journal

  • MICROBIOLOGY and IMMUNOLOGY

    MICROBIOLOGY and IMMUNOLOGY 43(7), 669-678, 1999-07-20

    Center For Academic Publications Japan

References:  32

Codes

  • NII Article ID (NAID)
    10004790496
  • NII NACSIS-CAT ID (NCID)
    AA00738350
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03855600
  • NDL Article ID
    4788312
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z53-M387
  • Data Source
    CJP  NDL  J-STAGE 
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