Stable Marker on Flagellin Gene Sequences Related to Arabinose Non-Assimilating Pathogenic Burkholderia pseudomallei

Access this Article

Search this Article

Author(s)

Abstract

Using PCR-based isolation and sequence analysis of the flagellin gene from two distinct biotypes of <i>Burkholderia pseudomallei</i>, a 15-bp deletion was found within the variable domain of the gene in isolates capable of assimilating arabinose (Ara+). This finding led to the development of a PCR-based method in order to differentiate and identify pathogenic <i>B. pseudomallei</i> for epidemiological study. A pair of specific primers was designed covering the 15-bp deletion region at the variable domain. PCR-amplification products of 176 and 191bp in size were detected from 41 Ara+ isolates and 39 Ara- isolates of <i>B. pseudomallei, </i> respectively. Moreover, flagellin gene fragments of other bacterial species tested in this study were not amplified using these primers. The results suggest that the fiagellin gene sequences of both <i>B. pseudomallei</i> biotypes in this region are stable and distinct. This method can be applied and useful for the epidemiological study of <i>B. pseudomallei</i>.

Journal

  • MICROBIOLOGY and IMMUNOLOGY

    MICROBIOLOGY and IMMUNOLOGY 43(11), 995-1001, 1999-11-20

    Center For Academic Publications Japan

References:  19

Codes

  • NII Article ID (NAID)
    10004791811
  • NII NACSIS-CAT ID (NCID)
    AA00738350
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    03855600
  • NDL Article ID
    4890169
  • NDL Source Classification
    ZS9(科学技術--医学--病理学・微生物学・寄生虫学・感染・免疫学・血清学・アレルギー)
  • NDL Call No.
    Z53-M387
  • Data Source
    CJP  NDL  J-STAGE 
Page Top