Amplification of SPR responses for the immunoreaction using anti HIV gp41 antibody by the method of molecular accumulation

• Nishimura Tomoaki

  Hiroshima Prefectural University

• Hifumi Emi

  Hiroshima Prefectural University

• Shimizu Kousuke

  Hiroshima Prefectural University

• Egashira Naoyoshi

  Hiroshima Prefectural University

• Uda Taizo

  Hiroshima Prefectural University

Abstract

Improvement of the detection limit of surface plasmon resonance (SPR) apparatus is an important issue when it is applied to immunosensor to detect the biological substances in serum and/or urine. In this study, we investigated two kinds of molecular accumulation methods. The method of consecutive accumulation, in which the second and third antibodies were successively bound onto the antigen (gp41 of human immunodeficiency virus-1), enhanced the SPR response to the anti gp41 antibody binding by about 7 fold. On the other hand, the method of complexed antibody accumulation, in which the second and third antibodies were simultaneously bound onto the antigen, gave the higher response by over 10 fold. In these methods, the appropriate second and third antibodies must be chosen in addition to determination of the preferable concentration.

Journal

• IEEJ Transactions on Sensors and Micromachines

  IEEJ Transactions on Sensors and Micromachines 119 (11), 522-527, 1999

  The Institute of Electrical Engineers of Japan

Keywords

• surface plasmon resonance
• biosensor
• immunoreaction
• sensitivity
• HIV
• gp41

Details

• CRID: 1390001204462649728
• NII Article ID: 10004834443
• NII Book ID: AN1052634X
• DOI: 10.1541/ieejsmas.119.522
• ISSN: 13475525
• ISSN: 13418939
• NDL BIB ID: 4892670
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4892670
• Web Site: http://www.jstage.jst.go.jp/article/ieejsmas1995/119/11/119_11_522/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed