Amplification of SPR responses for the immunoreaction using anti HIV gp41 antibody by the method of molecular accumulation
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- Nishimura Tomoaki
- Hiroshima Prefectural University
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- Hifumi Emi
- Hiroshima Prefectural University
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- Shimizu Kousuke
- Hiroshima Prefectural University
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- Egashira Naoyoshi
- Hiroshima Prefectural University
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- Uda Taizo
- Hiroshima Prefectural University
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Abstract
Improvement of the detection limit of surface plasmon resonance (SPR) apparatus is an important issue when it is applied to immunosensor to detect the biological substances in serum and/or urine. In this study, we investigated two kinds of molecular accumulation methods. The method of consecutive accumulation, in which the second and third antibodies were successively bound onto the antigen (gp41 of human immunodeficiency virus-1), enhanced the SPR response to the anti gp41 antibody binding by about 7 fold. On the other hand, the method of complexed antibody accumulation, in which the second and third antibodies were simultaneously bound onto the antigen, gave the higher response by over 10 fold. In these methods, the appropriate second and third antibodies must be chosen in addition to determination of the preferable concentration.
Journal
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- IEEJ Transactions on Sensors and Micromachines
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IEEJ Transactions on Sensors and Micromachines 119 (11), 522-527, 1999
The Institute of Electrical Engineers of Japan
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Details 詳細情報について
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- CRID
- 1390001204462649728
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- NII Article ID
- 10004834443
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- NII Book ID
- AN1052634X
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- ISSN
- 13475525
- 13418939
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- NDL BIB ID
- 4892670
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed