DNA固定化カーボンフェルトをリアクターとするDNA結合性薬物のフロー型バイオセンシング

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  • Flow-through Biosensing of DNA-binding Drugs using DNA-Immobilized Carbon Felt as a Reactor
  • DNA コテイカ カーボンフェルト オ リアクター ト スル DNA ケツゴウセイ ヤクブツ ノ フローガタ バイオセンシング

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Oxygen electrode-based flow-type amperometric mutant sensor was proposed using DNA-immobilized carbon felt as a sensing element for DNA-binding drugs. Catalytic activity of immobilized DNA-Cu (II) complex for air oxidation of aminoethanthiol (AET) was reversibly inhibited by intercalation with DNA binding drugs and antibiotics. Kinetic measurements indicate that quinacrine (QN) competitively inhibits the DNA-Cu (II) complex-catalyzed oxidation of AET. These phenomena could be utilized for development of novel flow-type biosensing system for DNA-binding drugs consisting with immobilized DNA-Cu (II) reactor and oxygen electrode detector. The peak-shape current responses were observed for various DNA-binding drugs, and the magnitude and the shape of the responses depend considerably on the kinds and concentrations of DNA-binding drugs.

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