Analysis of expressed sequence tags from a Japanese eel Anguilla japonica spleen cDNA library.

• MIYAHARA TAKAHIRO

  Laboratory of Genetics, Department of Aquatic Biosciences, Tokyo University of Fisheries

• HIRONO IKUO

  Laboratory of Genetics, Department of Aquatic Biosciences, Tokyo University of Fisheries

• AOKI TAKASHI

  Laboratory of Genetics, Department of Aquatic Biosciences, Tokyo University of Fisheries

抄録

We generated 196 expressed sequence tags (EST) from a Japanese eel Anguilla japonica spleen cDNA library. Of these, 90 EST (46%) showed no homology to known genes, while 106 EST (54%) showed homology to known genes and transcripts. Of the 106 homologous EST, 13 were homologous to 12 different immune-related genes, 46 were homologous to 33 different ribosomal proteins, three were homologous to the 28S rRNA gene, 10 were homologous to eight different mitochondrial genes and the remaining 34 encoded 28 different identified genes. Six identified EST are similar to previously reported EST of Japanese flounder Paralichthys olivaceus spleen cDNA. These analyses demonstrate the utility of EST and sequence-tagged clones in comparative studies of gene expression in the fish spleen.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 66 (2), 257-260, 2000

  公益社団法人 日本水産学会

キーワード

• Anguilla japonica
• cDNA
• eel
• expressed sequence tag
• spleen

詳細情報

• CRID: 1390282679405052672
• NII論文ID: 130003742247
• NII論文ID: 10004857894
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2000.00042.x
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 5381843
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5381843
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可