In Vitro Effects of 17α-hydroxyprogesterone and 17α,20β-dihydroxy-4-pregnen-3-one on Final Maturation of Oocytes at Various Developmental Stages in Artificially Matured Japanese Eel Anguilla japonica

• Kagawa Hirohiko

  National Research Institute of Aquaculture

• Tanaka Hideki

  National Research Institute of Aquaculture

• Ohta Hiromi

  National Research Institute of Aquaculture

• Okuzawa Koichi

  National Research Institute of Aquaculture

• Hirose Keiji

  National Research Institute of Fisheries Science

書誌事項

タイトル別名

• <i>In Vitro</i> Effects of 17α-hydroxyprogesterone and 17α, 20β-dihydroxy-4-pregnen-3-one on Final Maturation of Oocytes at Various Developmental Stages in Artificially Matured Japanese Eel <i>Anguilla japonica</i>
• In Vitro Effects of 17アルファ hydroxyproge
• <i>In Vitro</i> Effects of 17&alpha;-hydroxyprogesterone and 17&alpha;, 20&beta;-dihydroxy-4-pregnen-3-one on Final Maturation of Oocytes at Various Developmental Stages in Artificially Matured Japanese Eel <i>Anguilla japonica</i>
• In vitro effects of 17α-hydroxy progesterone and 17, 20β-dihydroxy-4-pregnen-3-one on final maturation of oocytes at various developmental stages in artificially matured Japanese eel (Anguilla japonica)

抄録

In vitro effects of 17 α-hydroxyprogesterone and 17 α, 20 β-dihydroxy-4-pregnen-3-one (DHP) on germinal vesicle breakdown (GVBD) of oocytes at various developmental stages were examined in artificially matured Japanese eel. Neither 17 α-hydroxyprogesterone nor DHP induced GVBD in oocytes at a tertiary yolk stage ranging from 600-700 μm in diameter. Oocytes at a migratory nucleus stage ranging from 700 to 800 μm in diameter underwent GVBD in response to 17 α-hydroxyprogesterone and DHP at concentrations of 10 and 100 ng/ml. Oocytes over 800 μm in diameter became more sensitive to the steroids. These results suggest that oocytes acquired the ability to respond to the maturation-inducing steroid at the migratory nucleus stage. Also, oocyte diameter and its microscopic features can be utilized as a reliable indicator of the induced maturation and ovulation by the injection of the steroids.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 61 (6), 1012-1015, 1995

  公益社団法人 日本水産学会

キーワード

• eel
• in vitro
• oocyte maturation
• steroid

詳細情報

• CRID: 1390001204430466944
• NII論文ID: 130003902818
• NII論文ID: 10004864416
• NII書誌ID: AA10993718
• DOI: 10.2331/fishsci.61.1012
• COI: 1:CAS:528:DyaK28XhvVKmtw%3D%3D
• NDL書誌ID: 3920579
• ISSN: 09199268
• ISSN: http://id.crossref.org/issn/09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3920579
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/61/6/61_6_1012/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可