Milt Production in the Japanese Eel Anguilla japonica Induced by Repeated Injections of Human Chorionic Gonadotropin

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  • Milt Production in the Japanese Eel <i>Anguilla japonica</i> Induced by Repeated Injections of Human Chorionic Gonadotropin
  • Milt Production in the Japanese Eel Ang

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Sexually immature male eels (253g average body weight), reared in sea water at 20°C, were injected weekly (14 times in total) with human chorionic gonadotropin (HCG) at a concentration of 250 or 750 IU/fish/week. Changes of the milt weight and milt quality were examined two days after each injection. Some of the treated males of both the 250 IU and 750 IU groups spermiated after the 5th injection, and most males spermiated after the 6th injection. In the 250 IU group, the milt weight gradually increased with increase in the repetitions of injection. It became constant (1-2g) during the period of the 11th-13th injection, and reached 4.0g after the 14th injection. Acquisition of potential for sperm motility was attained during the period of the 7th-9th injections and the percent motility of spermatozoa at 15 sec after dilution with 450mM NaCl remained at approximately 70% until after the 14 th injection. Milt pH (8.1-8.2) during the period of the 7th-9th injection was significantly higher than the 10th-11th injection period. Serum 17, 20β-dihydroxy-4-pregnen-3-one levels in the males of both groups remained low (0.1-0.2 ng/ml) throughout the experiment, although they were significantly higher than the saline control levels (0.05-0.08 ng/ml). In the 750 IU group, there was a marked difference in milt weight between individual males. The mean total milt weight throughout the experiment per fish of the 250 IU group was 9.4g, and that of the 750 IU group was 6.1g. The results indicate that repeated weekly injections of HCG at 250 IU/fish (about 1 IU/gBW) over 10 weeks is efficient for the artificial maturation of immature male eels.

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