Discrimination of Laver Cultivars with RAPD Markers

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  • Discrimination of Laver Cultivars with

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The cultivar discrimination of the laver (Porphyra yezoensis and P. tenera) was examined with random amplified polymorphic DNA (RAPD) analysis using purified genomic DNA and arbitrary primers. A total of 91 primers were used for the preliminary screening of RAPD primers, 18 of which were found to generate polymorphic PCR products (RAPD markers) among six cultivars. Eight of the 18 produced RAPD markers which were found in specific single cultivar and the other ten primers produced RAPD markers which were shared by more than two cultivars. Six cultivars could be distinguished by the combination of these RAPD markers. RAPD banding patterns were similar among independent lots of DNA extraction and among three thallus samples which were identical in cultivar but cultured and harvested in different years. These results suggested that RAPD markers were reproducible and stable during the culture of thalli and long-term propagation of concho elis, and therefore were genetically significant. RAPD analysis thus appears to be helpful in the discrimination of laver cultivars.

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