Glyceraldehyde 3-Phosphate Dehydrogenase, a Specific Sarcoplasmic Protein Insolubilized during Cold-storage of Sardine
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- Nakagawa Takayuki
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Ando Masashi
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Makinodan Yasuo
- Department of Fisheries, Faculty of Agriculture, Kinki University
書誌事項
- タイトル別名
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- Glyceraldehyde 3-Phosphate Dehydrogenas
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The changes in the extractability of the sarcoplasmic proteins from the ordinarymuscle of sardine during cold-storage were determined. The protein extractability from sardine decreased by 21% after three-day storage. SDS-gel electrophoresis showed that 36 kDa component from sardine muscle decreased by 61% during cold-storage. The 36 kDa component in the fresh muscle of sardine was purified and identified electrophoretically as glyceraldehyde 3-phosphate dehydrogenase. The sardine and yellowtail enzymes showed the highest activity at around pH 8.2, and were stable at pH 6-9. The heating temperature and time required for half inactivation was 47°C and 15min, respectively. Km values were 1.03mM for sardine and 1.56mM for yellowtail enzymes. On the other hand, the activity of sardine enzyme gradually decreased during cold-storage, whereas that of yellowtail enzyme rather increased up to three-day storage. This difference of the stability at 4°C coincided with the changes in the extractability of 36 kDa component detected in SDS-gel electrophoretic patterns.
収録刊行物
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- Ｆｉｓｈｅｒｉｅｓ ｓｃｉｅｎｃｅ
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Ｆｉｓｈｅｒｉｅｓ ｓｃｉｅｎｃｅ 62 (5), 766-771, 1996
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204428075776
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- NII論文ID
- 130003902920
- 10004867557
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4060759
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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