Purification and Properties of a Proteinaceous Toxin Newly Found in the Roe of Lamprey <i>Lampetra japonica</i>

• Shiomi Kazuo

  Department of Food Science and Technology, Tokyo University of Fisheries

• Miyauchi Koji

  Department of Food Science and Technology, Tokyo University of Fisheries Faculty of Bioresouces Sciences, Department of Marine Sciences and Resources, Nihon University

• Shimakura Kuniyoshi

  Department of Food Science and Technology, Tokyo University of Fisheries

• Nagashima Yuji

  Department of Food Science and Technology, Tokyo University of Fisheries

Bibliographic Information

Other Title

• Purification and Properties of a Proteinaceous Toxin Newly Found in the Roe of Lamprey Lampetra japonica
• Purification and Properties of a Protei

Abstract

The aqueous extract from the mature roe (gonad index>10%) of the lamprey Lampetra japonica was found to be lethal to mice when injected intravenously, while that from the immature roe(gonad index about 4%) was nontoxic. Dinogunellin, the well-known fish roe toxin, was notdetected in the lamprey roe. The lamprey toxin was a thermolabile, basic protein and purified to an electrophoretically pure state from the mature roe by chromatography on CM-cellulose and hydroxyapatite, FPLC on Mono S, and HPLC on TSKgel Ether-5PW. The purified toxin was highly lethal to miceupon intravenous injection; its LD₅₀ was estimated to be 13 μg/kg. Analyses by SDS-PAGE either with or without a reducing agent showed that the toxin has a molecular mass of 36 kDa composed of two kinds of subunits with molecular masses of 28 and 10 kDa. The amino acid composition of the toxin was characterized by the abundance of Ser, Glx, and Gly and the scarcity of half-Cys.

Journal

• Fisheries science

  Fisheries science 63 (1), 142-146, 1997

  The Japanese Society of Fisheries Science

Keywords

• proteinaceous toxin
• lamprey
• Lampetra japonica
• roe
• purification

Details

• CRID: 1390001204428544896
• NII Article ID: 130003903001
• NII Article ID: 10004868983
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.63.142
• NDL BIB ID: 4154003
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4154003
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/63/1/63_1_142/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed