Genetic Diversity of Five Strains of Red Sea Bream <i>Pagrus major</i> by RFLP Analysis of the mtDNA D-loop Region

• Tabata Kazuo

  Hyogo Prefectural Fisheries Experimental Station

• Kishioka Hideshi

  Department of Aquaculture, Faculty of Agriculture, Kochi University

• Takagi Motohiro

  Department of Aquaculture, Faculty of Agriculture, Kochi University

• Mizuta Akira

  Hyogo Prefectural Fisheries Experimental Station

• Taniguchi Nobuhiko

  Department of Aquaculture, Faculty of Agriculture, Kochi University

Bibliographic Information

Other Title

• Genetic Diversity of Five Strains of Red Sea Bream Pagrus major by RFLP Analysis of the mtDNA D-loop Region
• Genetic Diversity of Five Strains of Re

Abstract

Restriction fragment length polymorphism (RFLP) analysis of the mitochondrial DNA (mtDNA) D-loop region was used to compare the genetic diversity of 5 strains of red sea bream Pagrus major. Composite haplotypes, representing the information from 6 restriction endonucleases, were generated for samples of 35-40 fish from each strain. The components of haplotypes in the cultured strains differed considerably from those of the natural population. This is remarkable from the point of view of haplotype specialization. The results of a chi-square randomization test, in which differences between the strains were very significant, also showed that the specializations of haplotype were accelerated. Haplotypes occurred at a rate of 0.11 to 0.29 in the cultured strains, and 0.33 in the natural population. Haplotypic diversities were 0.34 to 0.84 in the cultured strains, and 0.86in the natural population. The UPGMA clustering, based on pure mean nucleotide sequence divergence, differed entirely from the UPGMA clustering based on the allozyme genetic distance. For this reason, it is thought that RFLP analysis of the mtDNA D-loop region could sensitively detect the bottleneck effect caused by a decrease in the effective number of parents. The pure mean nucleotide sequence divergences between the cultured strains were wider (17 times) and higher (29 times) than the values between the natural population. Thus, RFLP analysis of the mtDNA D-loop region was thought to be a good method for monitoring the genetic changes that occur during selective breeding.

Journal

• Fisheries science

  Fisheries science 63 (3), 344-348, 1997

  The Japanese Society of Fisheries Science

Keywords

• mtDNA
• D-loop
• RFLP
• genetic diversity
• cultured red sea bream
• red sea bream

Details

• CRID: 1390001204429372928
• NII Article ID: 130003903021
• NII Article ID: 10004869862
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.63.344
• NDL BIB ID: 4231314
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4231314
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/63/3/63_3_344/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed