Artificial Fertilization Using Testicular Spermatozoa in the Japanese Eel <i>Anguilla japonica</i>

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  • Artificial Fertilization Using Testicular Spermatozoa in the Japanese Eel Anguilla japonica
  • Artificial Fertilization Using Testicul

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Abstract

The motility and fertility of the Japanese eel testicular spermatozoa, which hadacquired motility during an incubation with K30 solution (consisting of 30mM KCl, 134.5 mM NaCl, 1.3mM CaCl2, 1.6mM MgCl2, 20mM NaHCO3, and buffered with 20mM TAPS-NaOH at pH 8.1), were compared with those of the milt spermatozoa obtained from the genital pore of a Japanese eel. The motility percentages of milt spermatozoa and testicular milt spermatozoa, were 84.7±4.1% and 2.6±1.9%, respectively. The percent motility of incubated testicular milt spermatozoa was 63.3±2.2%. The fertility and hatchability of the milt and incubated testicular milt were significantly higherthan those of testicular milt. There were no significant differences found between the fertility and hatchability of the milt and those of the incubated testicular milt. To examine the toxicity of the high potassium oncentration in the K 30 solution on egg fertility, we compared the fertility and hatchability ofmilt spermatozoa diluted with K 30 solution or K 15 solution. No significant differences in the fertility or hatchability were found between the K 30 and the K 15 solutions. These results indicate that the testicular spermatozoa which acquired motility during their incubation in K 30 solution are just as fertile as the motile milt spermatozoa, and that K 30 solution is suitable as a milt diluent in the artificial fertilization of the eel.

Journal

  • Fisheries science

    Fisheries science 63 (3), 393-396, 1997

    The Japanese Society of Fisheries Science

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