Chimeric Fish Produced by Exchanging Upper Parts of Blastoderms in Goldfish Blastulae

DOI Web Site Web Site 3 Citations 16 References
  • Yamaha Etsuro
    Nanae Fish Culture Experimental Station, Faculty of Fisheries, Hokkaido University Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University
  • Mizuno Toshiro
    Department of Molecular Biology, School of Science, Nagoya University
  • Hasebe Yutaka
    Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University
  • Yamazaki Fumio
    Laboratory of Breeding Sciences, Faculty of Fisheries, Hokkaido University

Bibliographic Information

Other Title
  • Chimeric Fish Produced by Exchanging Up

Search this article

Abstract

Chimeric fish were produced by exchanging the upper halves of blastoderms at theblastula stage between goldfish-common carp hybrids with pigmented scales and goldfish with clearscales, or between goldfish with clear scales and triploid crucian carp with pigmented scales. Blastoderm transplantation was carried out in Ringer's solution containing 1.6% albumen within one minute after isolation of the blastoderm. In the absence of albumen, the transplantation was not successful. The larvae and young fish were confirmed to be chimeric fish, as transplanted blastoderm cells could be detected histologically and as the fish exhibited phenotypes typical of both fish used to produce the chimeras. Blastomere mixing between the upper exchanged and lower halves of blastoderms occurred but notto a great extent, and transplanted cells from the upper halves of blastoderms labeled with biotin were mainly distributed in the head region.

Journal

  • Fisheries science

    Fisheries science 63 (4), 514-519, 1997

    The Japanese Society of Fisheries Science

Citations (3)*help

See more

References(16)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top