Isolation of Birnavirus from Agemaki(Jack Knife Clam)Sinonovacura constricta and Survey of the Virus Using PCR Technique

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  • Isolation of Birnavirus from Agemaki (Jack Knife Clam) <i>Sinonovacura constricta</i> and Survey of the Virus Using PCR Technique
  • Isolation of Birnavirus from Agemaki Ja

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Two strains of birnavirus were isolated from Agemaki (jack knife clam) Sinonovacula constricta originating in the Ariake sea, Japan, and from imported shells from Korea. The genome sequence of the VP 2/Ns junction regions of virus genome revealed that the 2 strains are verysimilar to each other and also similar to other birnaviruses isolated from several marine fishes. Routine survey of the virus genome from Agemaki by PCR was performed in 1993 and 1994. The birnavirus genome was detected in high ratios using samples recollected from several fishing grounds where Korean shells were transplanted. The imported shells from Korea also possessed the virus genome. These findings suggest that birnavirus is widely present in Agemaki in the Ariake sea and on the Korean coast.

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