Detection and Identification of Infectious Hematopoietic Necrosis Virus (IHNV) by Reverse Transcription (RT)-polymerase Chain Reaction (PCR)

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  • Yoshinaka Toko
    Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University
  • Yoshimizu Mamoru
    Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University
  • Sawabe Tomoo
    Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University
  • Ezura Yoshio
    Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University

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  • Detection and Identification of Infecti

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Abstract

A method based on reverse transcription (RT)-polymerase chain reaction (PCR) wasestablished for detection and identification of infectious hematopoietic necrosis virus (IHNV). A set of primers was prepared for amplification of a 510 bp nucleotide which encodes a region of IHNV nucleoprotein (N) gene. The PCR product was confirmed as the IHNV specific nucleotide by southern hybridization with an oligonucleotide probe synthesized from the N gene. The PCR was able to amplify the target sequence from five representative strains of IHNV from Japan and North America. There was no PCR product from five other fish rhabdoviruses. Viruses from ovarian fluid of masu salmon collected at Shibetsu River, Ichani River in Hokkaido and kidney tissue of rainbow trout in Aichi Prefecture were isolated and identified using the RT-PCR.

Journal

  • Fisheries science

    Fisheries science 63 (4), 592-595, 1997

    The Japanese Society of Fisheries Science

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