Aggregation of Actin and Myosin in Carp Myofibrils during Frozen Storage under Phosphate Buffer Conditions
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- Kitazawa Hiroyuki
- Faculty of Fisheries, Hokkaido University
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- Kawai Yuji
- Faculty of Fisheries, Hokkaido University
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- Yamazaki Koji
- Faculty of Fisheries, Hokkaido University
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- Inoue Norio
- Faculty of Fisheries, Hokkaido University
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- Shinano Haruo
- Faculty of Fisheries, Hokkaido University
書誌事項
- タイトル別名
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- Aggregation of Actin and Myosin in Carp
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抄録
Carp myofibrils suspended in 5 and 50mM phosphate buffer (pH 6.8) were stored at -4, -11, and -26°C for 167 days, and the changes in volume of myofibrillar protein components in salt-soluble and salt-insoluble fractions were followed by polyacrylamide gel electrophoresis. The extents of the decrease of actin and myosin heavy chain components in the salt-soluble fraction and the increase of those in the salt-insoluble fraction were greater, and the insolubilization of those components was accelerated with an increase in storage temperature at both buffer concentrations. Under all storage conditions, there was a transient steady stage during which the changes of actin and myosin heavy chain components were very small in each fraction. The steady stages in 5 and 50mM phosphate buffers occurred at storage periods 10-89 (or 10-40) and 40-89, respectively, and especially those of the actin component in the salt-insoluble fraction were evident. The beginning of the steady stage in 5mM phosphate buffer was earlier than that in 50mM. It seems that the myofibrils were insolubilized as the actin-myosin complex in the storage range of 0-23 days at -4°C in 5mM phosphate buffer and in the range of 0-89 days under all other storage conditions.
収録刊行物
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- Fisheries science
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Fisheries science 63 (4), 635-638, 1997
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204428789376
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- NII論文ID
- 130003903073
- 10004871044
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- NII書誌ID
- AA10993718
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- NDL書誌ID
- 4277919
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- ISSN
- 09199268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可