Amino Acid Sequences of Kunitz-type Protease Inhibitors from the Sea Anemone <i>Actinia equina</i>

• Ishida Masami

  Department of Food Science and Technology, Tokyo University of Fisheries

• Minagawa Sonomi

  Department of Food Science and Technology, Tokyo University of Fisheries

• Miyauchi Koji

  Department of Food Science and Technology, Tokyo University of Fisheries Faculty of Bioresources Sciences, Department of Marine Sciences and Resources, Nihon University

• Shimakura Kuniyoshi

  Department of Food Science and Technology, Tokyo University of Fisheries

• Nagashima Yuji

  Department of Food Science and Technology, Tokyo University of Fisheries

• Shiomi Kazuo

  Department of Food Science and Technology, Tokyo University of Fisheries

Bibliographic Information

Other Title

• Amino Acid Sequences of Kunitz-type Protease Inhibitors from the Sea Anemone Actinia equina
• Amino Acid Sequences of Kunitz-type Pro

Abstract

Four serine protease inhibitors (AEPI-I, II, III and IV) were isolated from the sea anemone Actinia equina by a slight modification of our previous method. When the native inhibitors were applied to a sequencer, 36, 36, 35, and 37 amino acid residues from the N-terminus were identified for AEPI-I, II, III, and IV, respectively. The remaining sequences of AEPI-I and II were deduced from analyses of peptide fragments obtained by digestion of S-carboxamidomethylated molecules with either V8 protease or lysyl endopeptidase. Both inhibitors were composed of 59 amino acid residues including 6 half-Cys residues and their sequences were very similar to each other with replacements at only two positions. The positions of half-Cys residues and the entire-chain homology identified these inhibitors as members of the Kunitz-type family. Notably, the sequences of the two contact sites with serine proteases were highly conserved within the sea anemone inhibitors.

Journal

• Fisheries science

  Fisheries science 63 (5), 794-798, 1997

  The Japanese Society of Fisheries Science

Keywords

• sea anemone
• Actinia equina
• serine protease inhibitors
• Kunitz-type protease inhibitors
• amino acid sequence

Details

• CRID: 1390001204430296192
• NII Article ID: 130003903108
• NII Article ID: 10004871688
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.63.794
• NDL BIB ID: 4326635
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4326635
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/63/5/63_5_794/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed