Thermal Unfolding of the Cold-acclimated Type of Carp Light Meromyosin Expressed by Recombinant DNA in <i>Escherichia coli</i>

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  • Kakinuma Makoto
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Funabara Daisuke
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Nakaya Misako
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Hirayama Yasushi
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Watabe Shugo
    Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Maeda Kayo
    International Institute for Advanced Research, Matsushita Electric Industrial Co., Ltd.
  • Ooi Tatsuo
    Department of Food Science, Kyoto Women's University

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Other Title
  • Thermal Unfolding of the Cold-acclimated Type of Carp Light Meromyosin Expressed by Recombinant DNA in Escherichia coli
  • Thermal Unfolding of the Cold-acclimate

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Abstract

cDNA encoding fast skeletal muscle light meromyosin (LMM) predominantly expressed in carp acclimated to a cold temperature of 10°C was inserted into an Escherichia coli expression vector pET-11a. The resulting plasmid pET10 produced non-fused carp 10°C-type LMM, yielding 10% of the total proteins in E. coli. The 10°C-type LMM was purified by altered dialyses against high- and low-ionic-strength buffers and ion-exchange chromatography. An apparent molecular mass of the purified LMM was about 74, 000 on SDS-PAGE, which was slightly larger than that previously reported for LMM isolated from carp acclimated to 10°C. Transition temperatures (Tm) were 30.2 and 34.9°C for the present 10°C-type LMM on DSC analysis. This LMM exhibited a typical pattern of α-helix in CD spectroscopy with two minima at 222 and 208 nm, and its α-helical content at 20°C was about 70%. The maximal decreasing rate derivative at 35°C of the mean residue ellipticity of carp LMM per unit change of measuring temperature well reflected Tm values observed in DSC analysis.

Journal

  • Fisheries science

    Fisheries science 63 (6), 1008-1013, 1997

    The Japanese Society of Fisheries Science

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