Detection of Heat Stability Variants in GPI Isozymes of Goldfish and Crucian Carp

• Takase Takeharu

  Faculty of Agriculture, Tohoku University

• Kijima Akihiro

  Faculty of Agriculture, Tohoku University

• Fujio Yoshihisa

  Faculty of Agriculture, Tohoku University

Bibliographic Information

Other Title

• Detection of Heat Stability Variants in

Abstract

A combination of electrophoretic and heat denaturation techniques was used to identify the gene loci and alleles of the isozymes in goldfish and crucian carp. Heat denaturation experiments revealed different heat stabilities among three loci (Gpi-1^*, Gpi-2^*, and Gpi-3^*) encoding glucosephosphate isomerase (GPI) in goldfish and crucian carp. The heat stability differences were found in all the three loci examined. Cross experiments indicated that the observed differences among alleles at each locus in heat stability are controlled by a single incomplete dominant gene. Moreover, the heat stability alleles demonstrated a divergence in Gpi-1^* and Gpi-2^* loci between goldfish and crucian carp. The heat denaturation technique was thus useful for the revelation of hidden genetic variations of isozymes.

Journal

• Fisheries science

  Fisheries science 64 (1), 31-34, 1998

  The Japanese Society of Fisheries Science

Keywords

• goldfish
• crucian carp
• glucosephosphate isomerase
• isozyme
• heat stability allele

Details

• CRID: 1390282679406316416
• NII Article ID: 130003742109
• NII Article ID: 10004872980
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.31
• NDL BIB ID: 4406499
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4406499
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/1/64_1_31/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed