Cryopreservation of Nauplius Larvae of the Barnacle, <i>Balanus amphitrite</i> Darwin

• Khin-Maung-Oo

  Fisheries Laboratory, Faculty of Agriculture, The University of Tokyo

• Kurokura Hisashi

  Laboratory of Global Fisheries Science, Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Iwano Tsuyoshi

  School of Fisheries Sciences, Kitasato University

• Okamoto Ken

  Fisheries Laboratory, Faculty of Agriculture, The University of Tokyo

• Kado Ryusuke

  School of Fisheries Sciences, Kitasato University

• Hino Akinori

  Fisheries Laboratory, Faculty of Agriculture, The University of Tokyo

Bibliographic Information

Other Title

• Cryopreservation of Nauplius Larvae of the Barnacle,Balanus amphitrite Darwin

Abstract

The investigation was conducted to develop the prospects for application of cryopreservation technique to nauplius larvae of economically important crustacean, such as Japanese prawns or crabs. Since Balanus amphitrite possesses naupliar stages similar to those of prawn, the nauplius-II larvae of barnacle were applied in this experiment. The larvae were equilibrated for 20 minutes in a cryoprotectant solution containing 1.5M dimethyl sulfoxide (DMSO) in 29‰ filtered seawater. They were then frozen to-196°C by a two-step freezing procedure. The optimum conditions for the cryopreservation of the nauplius-II larvae were: from ice seeding temperature of-12°C to the first step cooling temperature of-30°C was by a cooling rate of 0.5°C/min, then rapidly frozen to-196°C. Frozen nauplii were thawed at 50°C, and incubated in 29‰ filtered seawater after washing cryoprotectant. The diatom Chaetoceros calcitrans at a concentration of 40×10⁴ cells/ml was provided as food in the rearing of nauplii. After 28 days preservation in liquid nitrogen (-196°C), 78% of the larvae recovered and 19.8% of the larvae metamorphosed to cyprid.

Journal

• Fisheries science

  Fisheries science 64 (6), 857-860, 1998

  The Japanese Society of Fisheries Science

Keywords

• cryopreservation
• dimethyl sulfoxide
• nauplius-II
• cyprid
• barnacle

Details

• CRID: 1390282679405064704
• NII Article ID: 130003903261
• NII Article ID: 10004876579
• NII Book ID: AA10993718
• DOI: 10.2331/fishsci.64.857
• NDL BIB ID: 106839
• ISSN: 09199268
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I106839
• Web Site: https://www.jstage.jst.go.jp/article/fishsci1994/64/6/64_6_857/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed