Cloning and Sequencing of the Klebsiella K-36 astA Gene, Encoding an Arylsulfate Sulfotransferase

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Abstract

A gene-encoding arylsulfate sulfotransferase (ASST) was cloned from a <i>Klebsiella</i> K-36 genomic library. ASST tranfers a sulfate group from phenolic sulfate esters to a phenolic acceptor substrate. The gene, designated <i>astA</i>, was subcloned into vector pGEM3Zf(-) and sequenced. Recombinant clone-harbouring <i>astA</i> was directly identified using a fluorescent product. The nucleotide sequencing revealed an open reading frame (ORF) of 2, 082 by encoding a protein of 694 amino acids with a secretory signal sequence. A protein of similar size was visualized after <i>in vitro</i> transcription and translation using a plasmid carrying the cloned 3.1-kb fragment as a template. The N-terminal amino acid sequence of the purified processed protein was found to be identical to that predicted from the gene sequence. When searching the database for <i>astA</i> nucleotide or its deduced amino acid sequence, no significant homology to any sequence was found.

Journal

  • MICROBIOLOGY and IMMUNOLOGY

    MICROBIOLOGY and IMMUNOLOGY 40(8), 531-537, 1996-08-20

    Center For Academic Publications Japan

References:  29

Cited by:  4

Codes

  • NII Article ID (NAID)
    10004893476
  • NII NACSIS-CAT ID (NCID)
    AA00738350
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    03855600
  • Data Source
    CJP  CJPref  J-STAGE 
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