Investigation of IFN Type-I Receptor and IFN Regulatory Factor Expression Relating to Induction of 2', 5'-Oligoadenylate Synthetase in Cells Persistently Infected with the Mumps Virus

• FUJII Nobuhiro

  Department of Microbiology, Sapporo Medical University School of Medicine

• YOKOSAWA Noriko

  Department of Microbiology, Sapporo Medical University School of Medicine

• ISHIDA Setsuko

  Cancer Research Institute, Sapporo Medical University School of Medicine

• SHIRAKAWA Sachiko

  Department of Microbiology, Sapporo Medical University School of Medicine

• KUBOTA Toru

  Department of Microbiology, Sapporo Medical University School of Medicine

• INDOH Tomokazu

  Department of Microbiology, Sapporo Medical University School of Medicine

• FUJINAGA Kei

  Cancer Research Institute, Sapporo Medical University School of Medicine

• YASHIKI Teruo

  Laboratory of Technology, College of Medical Technology, Hokkaido University

Abstract

Poor induction of interferon-induced 2', 5'-oligoadenylate synthetase (2-5AS) activity has been demonstrated in cells persistently infected with the mumps virus or human T-lymphotropic virus type-I (HTLV-I). The suppression of 2-5AS induction is the result of the repression of 2-5AS gene expression at the transcription level. In a general way, after the binding of interferon-α (IFN-α) to cell surface-specific receptors, expression of 2-5AS gene is thought to be regulated by some transacting factors, IFN-regulatory factors (IRF-1 and IRF-2) and the IFN-stimulated gene factor (ISGF-3, a complex consisting of STAT 1α, STAT-2 and p48). To clarify the cause of the suppression mechanism(s), fluctuation in the number of IFN receptors and the levels of mRNAs in both IRF-1 and IRF-2 were examined in cells persistently infected with the mumps virus (FLMT and KBMT). There were few defferences in the number of IFN receptors and the level of IRF-2 mRNA between persistently infected cells and uninfected control cells. After the treatment of cells with INF, a slight reduction of IRF-1 mRNA was found in persistently infected cells as compared with that of the uninfected control cells.

Journal

• MICROBIOLOGY and IMMUNOLOGY

  MICROBIOLOGY and IMMUNOLOGY 40 (10), 777-781, 1996-10-20

  Center For Academic Publications Japan

Keywords

• 2', 5'-Oligoadenylate synthetase(2-5AS)
• Interferon
• Interferon receptor
• RT-PCR
• IRF-1
• IRF-2
• Mumps virus
• Persistent infection

Details

• CRID: 1573105974091935232
• NII Article ID: 10004894548
• NII Book ID: AA00738350
• ISSN: 03855600
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK2sXhtFSr&md5=e499701b40972ef168664445b251f29d
• Web Site: https://search.jamas.or.jp/link/ui/1997042218
• Text Lang: en
• Data Source:
  • CiNii Articles