Expression of intercellular adhesion molecule-1 on cultured glomerular endothelial cells by pro-inflammatory cytokines and lipopolysaccharide.
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- NITTA Kosaku
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- YUMURA Wako
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- UCHIDA Keiko
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- MIWA Naoko
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- HORITA Shigeru
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- TSUTSUI Takaaki
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- KAWASHIMA Akira
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
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- NIHEI Hiroshi
- Department of Medicine, Kidney Center, Tokyo Women's Medical College
抄録
Infiltration of leukocytes and mononuclear cells into the glomeruli is an early pathological finding in human and experimental glomerulonephritis. However, the cellular and molecular basis for cell infiltration into the glomeruli is not yet completely understood. In addition, there is little information on the expression of intercellular adhesion molecule-1(ICAM-1) on glomerular cells. In the present study, we investigated the expression of ICAM-1 on cultured bovine glomerular endothelial cells (GEN) and its regulation by the pro-inflammatory cytokines, interleukin-1β(IL-1β), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1) and lipopolysaccharide (LPS). Immunocytochemical staining showed that ICAM-1 molecules were constitutively expressed on the surface of GEN. In flow cytometric and ELISA analyses, ICAM-1 molecule expression on GEN was significantly upregulated by IL-1β, MCP-1 and LPS in a dose-dependent manner, but not by IL-6. LPS was the most potent inducer of ICAM-1 molecule expression on GEN. The effects of IL-1β, MCP-1 and LPS were observed as early as 4 h and reached a maximal level by 18h. These results suggest that ICAM-1 on GEN can participate in the infiltration of mononuclear cells into glomeruli in human and experimental glomerulonephritis.
収録刊行物
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- 日本腎臓学会誌
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日本腎臓学会誌 37 (2), 100-104, 1995
社団法人 日本腎臓学会