高脂肪食および抗酸化剤ＢＯ６５３のラット腎虚血再潅流障害に及ぼす影響

• 小柴 健一郎

  北里大学医学部麻酔科

• 熊野 和雄

  北里大学医学部泌尿器科

• 渡辺 刀美乃

  北里大学医学部生化学科

• 高嶋 佳昭

  中外製薬富士御殿場研究所

• 進士 修

  中外製薬富士御殿場研究所

書誌事項

タイトル別名

• Effects of high fat diet and a novel antioxidant (BO653) on ischemia reperfusion injury of rat kidney.

抄録

The effects of a high fat diet (HFD) on renal function, renal mitochondrial function and intrarenal oxygen-free radical scavenging activity were examined on the ischemia-reperfusion model of the rat kidney. Whether or not a novel lipophilic antioxidant (BO653) could minimize this effect in vivo was also investigated. Thirty minutes renal ischemia was introduced by vascular clamp in rats with or without HFD (choresterol 1.25%) . Some of the HFD rats received BO653 by gastric gavage. Creatinine clearance (Ccr) was measured 24 hours following the injury. Mitochondrial oxygen consumption and thiobarbituric acid reactive substance (TBARS), superoxide dismutase (SOD), glutathione peroxidase (GPX) and α-toco-pherol were measured in the kidney before, 30 min ischemia and 30 min after reperfusion. HFD significantly reduced Ccr after ischemia-reperfusion (45% decreased compared to normal diet), which was ameliorated by BO653. Thirty-minute ischemia deteriorated the mitochondrial function in the normal diet (ND) group, high fat diet (HFD) group and high fat diet+BO653 (HFD+BO) group. Thirty-minute reper-fusion ameliorated the mitochondrial function in all those groups. The kidney content of TBARS was not increased after the ischemia-reperfusion in all these groups. In the HFD group, the kidney content of GPX was higher than in the ND group during ischemia-reperfusion, but in the HFD group, the kidney content of SOD was significantly decreased after the thirty-minute ischemia. Thirty-minute ischemia decreased the kidney content of α-tocopherol in the HFD group, which was recovered by the thirty-minute reperfusion. In conclusion, a high fat diet deteriorates ischemia-reperfusion injury of the rat kidney and BO653 ameliorated this effect judged by creatinine clearance and renal mitochondrial function. Reperfusion injury could not be confirmed in the present model based on the results of lipid peroxidation and oxygen-free radical scavenging enzyme activity.

収録刊行物

• 日本腎臓学会誌

  日本腎臓学会誌 39 (5), 455-463, 1997

  社団法人 日本腎臓学会

キーワード

• ischemia reperfusion injury, high-fat diet, mitochondrial function, antioxidant therapy, lipid peroxidation

詳細情報

• CRID: 1390282679834639232
• NII論文ID: 130004170400
• NII論文ID: 10024976858
• NII論文ID: 10004919504
• NII書誌ID: AN10131749
• DOI: 10.14842/jpnjnephrol1959.39.455
• ISSN: 18840728
• ISSN: 03852385
• PubMed: 9283210
• Web Site: https://search.jamas.or.jp/link/ui/1998012386
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可