Amperometric Biosensors for Sensing of Hydrogen Peroxide Based on Electron Transfer between Horseradish Peroxidase and Ferrocene as a Mediator.

• NAKABAYASHI Yasuo

  Faculty of Engineering, Kansai University HRC, Kansai University

• YOSHIKAWA Hajime

  Faculty of Engineering, Kansai University

Abstract

A non-conducting polymer film of 3-aminophenol, in which horseradish peroxidase was immobilized, has been prepared on a carbon paste electrode containing both ferrocene as a mediator and horseradish peroxidase by the electrochemical polymerization. The response currents of this hydrogen peroxide sensor linearly increased with increasing the concentration of hydrogen peroxide of 0.6 to 20 μmol dm^-3; the straight line had a slope equal to 10.1 mA mol^-1 dm³ (r= 0.999). This hydrogen peroxide sensor may be available for the fabrication of other biosensors, e.g. glucose and cholesterol sensors, which are not influenced by easily oxidizable species, L-ascorbic acid and uric acid.

Journal

• Analytical Sciences

  Analytical Sciences 16 (6), 609-613, 2000

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679233665280
• NII Article ID: 10004955098
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.16.609
• COI: 1:CAS:528:DC%2BD3cXksVOit78%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 5380264
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5380264
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.16.609.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.16.609/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/16/6/16_6_609/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed