培養唾液腺細胞の移植に関する研究　移植腺細胞の機能と形態形成

DOI 被引用文献4件 参考文献19件
  • 桑原 佐和子
    中部労災病院歯科口腔外科
  • 畠 賢一郎
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座
  • 各務 秀明
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座
  • 鷲見 幸男
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座
  • 水野 裕和
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座
  • 山田 陽一
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座
  • 上田 実
    名古屋大学大学院医学研究科頭頸部感覚器外科学講座

書誌事項

タイトル別名
  • A study of transplantation of cultured salivary gland cells: Function and morphogenesis of transplanted cells.
  • Function and morphogenesis of transplanted cells
  • 移植腺細胞の機能と形態形成

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抄録

We have described culture methods for normal salivary gland cells, using 3 T 3 cells as a feeder layer. These cultured salivary gland cells are morphologically and biologically similar to normal salivary gland cells. However, the properties of cells cultured in vivo are unknown. In this paper, the second passage of cultured salivary gland cells was applied to atelocollagen sponges, which were then transplanted to the back of nude mice.<BR>The mice with transplanted cells showed liquid accumulation on the back, whereas the control animals did not.<BR>The liquid was viscous, faint brown in color, and contained amylase (mean, 2175 IU/l).<BR>Immunohistochemical staining showed that approximately 50 percent of transplanted cells were amylase positive 24 to 48 hours after transplantation. Four weeks after transplantation, the atelo collagen sponge was completely absorbed, and epithelial cells were distributed in a band-like fashion. The transplanted cells did not show ductal or acinar formation. Our results show that salivary gland cells cultured by our method retain the characteristics of salivary gland cells in vivo.<BR>It may be necessary to establish a novel in vivo model of salivary glands by using key growth factors.

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