Cloning and sequence of the SCS2 Gene, Which Can Suppress the Defect of INO1 Exopression in an Inositol Auxotrophic Mutant of Saccharomyces cerevisiae

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Author(s)

    • NIKAWA Jun-ichi
    • Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology
    • MURAKAMI Akiko
    • Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology
    • ESUMI Etsuko
    • Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology
    • HOSAKA Kohei
    • Department of Biochemistry, Grunma University School of Medicine

Abstract

<i>Saccharomyces cerevisiae ire15</i> mutant has a defect in the expression of <i>INO1</i>, showing the inositol auxotrophic phenotype [Nikawa, J. (1994) <i>Gene</i> 149, 367-372]. We have isolated five yeast genes which suppress the <i>ire15</i> mutation in multiple copies by genetic complementation. Among them, one gene, designated as <i>SCS2</i>, also suppressed the cholinesensitive dominant mutation, <i>CSE1</i> [Hosaka, K. <i>et al</i>. (1992) <i>J. Biochem</i>. 111, 352-358]. The <i>CSE1</i> mutation is not allelic to <i>ire15</i>. Sequencing analysis revealed that the <i>SCS2</i> gene encodes 244 amino acid residues with a calculated molecular mass of 26, 866. <i>INO2</i>/<i>SCS1</i>, which is another suppressor gene for <i>CSE1</i> and is known to be a positive factor for <i>INO1</i> expression, also suppressed the growth defect of the <i>ire15</i> mutant. These results clearly indicate that the <i>ire15</i> and <i>CSEI</i> mutations genetically interact and the <i>SCS2</i> and <i>INO2</i>/<i>SCS1</i> genes are involved in the regulation of <i>INO1</i> expression.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 118(1), 39-45, 1995-07-01

    The Japanese Biochemical Society

References:  37

Cited by:  3

Codes

  • NII Article ID (NAID)
    10005182440
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • Data Source
    CJP  CJPref  J-STAGE 
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