Enhancement of Stress- Induced Synthesis of Stress Proteins by Mastoparan in C6 Rat Glioma Cells^1

• KATO Kanefusa

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• ITO Hidenori

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• HASEGAWA Kaori

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• INAGUMA Yutaka

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• SUZUKI Atsushi

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• KOZAWA Osamu

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

• ASANO Tomiko

  Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center

抄録

The levels of two small stress proteins, hsp27 and αB crystallin, were low in C6 glioma cells confluency. However, the levels of the two proteins increased after exposure of cells to heat (42°C for 30 min) or arsenite (50-100μM for 1 h) stress. When cells were exposed to arsenite or heat in the presence of mastoparan, a peptide toxin from wasp venom, the induction of hsp27 and αB crystallin was markedly stimulated, as detected by means of specific immunoassays, Western blot analysis, and Northern blot analysis. The response of hsp70 to each stress was also enhanced in the presence of mastoparan. Treatment of cells with 40μM mastoparan alone barely induced the accumulation of hsp27 and αB crystallin. The stimulatory effect of mastoparan was little affected in cells that had been treated with pertussis toxin, but it was strongly suppressed in the presence of quinacrine, an inhibitor of phospholipase A₂. These results suggest that mastoparan, which is an activator of phospholipase A₂, enhances the responses to stress of hsp27, αB crystallin and hsp70 by increasing the metabolic activity of the arachidonic acid cascade.

収録刊行物

• The journal of biochemistry

  The journal of biochemistry 118 (1), 149-153, 1995-07-01

  社団法人 日本生化学会

キーワード

• crystallin
• heat- shock protein
• hsp27
• mastoparan
• stress protein

詳細情報

• CRID: 1571135649354878336
• NII論文ID: 10005182976
• NII書誌ID: AA00694073
• ISSN: 0021924X
• Web Site: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8537304&dopt=AbstractPlus
• Web Site: https://search.jamas.or.jp/link/ui/1996057323
• 本文言語コード: en
• データソース種別:
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