Morphology of Ras- Transformed Cells Becomes Apparently Normal Again with Tyrosine Kinase Inhibitors without a Decrease in the Ras- GTP Complex^1

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Author(s)

    • MUROYA Kenkoh
    • Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry
    • HATTORI Seisuke
    • Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry
    • NISHIDA Eisuke
    • Department of Genetics and Molecular Biology, Institute for Virus Research, Kyoto University
    • FUKUI Yasuhisa
    • Department of Applied Biological Chemistry The University of Tokyo

Abstract

Radicicol, an inhibitor of protein-tyrosine kinase, was found to cause morphological reversion of v-Ha-<i>ras</i>-transformed NIH3T3 fibroblasts and T24 human urinary bladder carcinoma cells that contain an activated ras mutation. The network of actin stress fibers was restored during the treatment with radicicol. A similar morphological change was observed with another protein-tyrosine kinase inhibitor, herbimycin A. Radicicol did not cause any changes in the proportion of the active GTP binding form of p21<sup>ras</sup> or, its subcellular localization. These results rule out the possibility that the morphological reversion by radicicol is due to direct or indirect inhibition of the p21<sup>ras</sup> function. Cycloheximide and actinomycin D inhibited the morphological change by radicicol, suggesting that the induced transcription of a gene (s) followed by <i>de novo</i> protein synthesis is required for suppression of the transformed phenotype in <i>ras</i>-transformed cells by tyrosine kinase inhibitors.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 118(1), 221-228, 1995-07-01

    The Japanese Biochemical Society

References:  43

Cited by:  3

Codes

  • NII Article ID (NAID)
    10005183336
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • Data Source
    CJP  CJPref  J-STAGE 
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