Morphology of Ras- Transformed Cells Becomes Apparently Normal Again with Tyrosine Kinase Inhibitors without a Decrease in the Ras- GTP Complex^1

• KWON Ho Jeong

  Department of Biotechnology The University of Tokyo

• YOSHIDA Minoru

  Department of Biotechnology The University of Tokyo

• MUROYA Kenkoh

  Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry

• HATTORI Seisuke

  Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, National Center of Neurology and Psychiatry

• NISHIDA Eisuke

  Department of Genetics and Molecular Biology, Institute for Virus Research, Kyoto University

• FUKUI Yasuhisa

  Department of Applied Biological Chemistry The University of Tokyo

• BEPPU Teruhiko

  Department of Biotechnology The University of Tokyo

• HORINOUCHI Sueharu

  Department of Biotechnology The University of Tokyo

抄録

Radicicol, an inhibitor of protein-tyrosine kinase, was found to cause morphological reversion of v-Ha-ras-transformed NIH3T3 fibroblasts and T24 human urinary bladder carcinoma cells that contain an activated ras mutation. The network of actin stress fibers was restored during the treatment with radicicol. A similar morphological change was observed with another protein-tyrosine kinase inhibitor, herbimycin A. Radicicol did not cause any changes in the proportion of the active GTP binding form of p21^ras or, its subcellular localization. These results rule out the possibility that the morphological reversion by radicicol is due to direct or indirect inhibition of the p21^ras function. Cycloheximide and actinomycin D inhibited the morphological change by radicicol, suggesting that the induced transcription of a gene (s) followed by de novo protein synthesis is required for suppression of the transformed phenotype in ras-transformed cells by tyrosine kinase inhibitors.

収録刊行物

• The journal of biochemistry

  The journal of biochemistry 118 (1), 221-228, 1995-07-01

  社団法人 日本生化学会

キーワード

• protein-tyrosine kinase
• radicicol
• Ras, Src
• transformation

詳細情報

• CRID: 1573950399121867776
• NII論文ID: 10005183336
• NII書誌ID: AA00694073
• ISSN: 0021924X
• Web Site: http://chemport.cas.org/cgi-bin/sdcgi?APP=ftslink&action=reflink&origin=jstage2&version=1.0&coi=1:CAS:528:DyaK2MXnsFegtL4%3D&md5=f40a1395bb77cc616afaa87907430f21
• Web Site: https://search.jamas.or.jp/link/ui/1996044221
• 本文言語コード: en
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