Nucleotide Sequence of the Uricase Gene from Bacillus sp. TB-90^1

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Abstract

The nucleotide sequence of the uricase gene from the thermophilic bacterium <i>Bacillus</i> sp. TB-90 was determined. The primary structure of the uricase deduced from the nucleotide sequence comprised 332 amino acids, with a total molecular mass of 37, 994 Da. The molecular mass of the subunit of the uricase produced by the transformant of <i>Escherichia coli</i> agreed well with this value. However, the molecular mass of a subunit of the uricase produced by <i>Bacillus</i> sp. TB-90 was found to be 34, 000 Da by SDS-PAGE. The difference between these molecular masses was attributed to processing of the C-terminal 13 amino acid residue in <i>Bacillus</i> sp. TB-90. Comparison of the enzymatic properties of both uricases showed that the thermostability of the uricase produced by the transformant was enhanced by about 10°C in comparison to that produced by <i>Bacillus</i> sp. TB-90.

Journal

  • The Journal of Biochemistry

    The Journal of Biochemistry 119(1), 80-84, 1996-01-01

    The Japanese Biochemical Society

References:  21

Cited by:  3

Codes

  • NII Article ID (NAID)
    10005185025
  • NII NACSIS-CAT ID (NCID)
    AA00694073
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021924X
  • Data Source
    CJP  CJPref  J-STAGE 
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